Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Nicotinic acid Size:500g 4 Riboflavin 0

4 Riboflavin 0

0 Beef Extract 5

2 at 25°C Suspend 28

at this strength the ph is around 6

VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Nicotinic acid Size:500g 4 Riboflavin 0Nicotinic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of nicotinic acid or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room