Terrific Broth Size:6 x 1kg Suspend 37gram powdered medium in

Suspend 37gram powdered medium in 1 litre distilled water

Tryptone 8 Yeast extract 5 NaCl 5 Agar 15 Final pH 7

9% Purity (TLC) One Spot IR Conform standard Solubility (5%

Glucose 11000 g/l KH2PO4 680

Karl Friehs and Erwin Flaschel

Terrific Broth Size:6 x 1kg Suspend 37gram powdered medium inTerrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E. coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium. Terrific Broth medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid. Tryptone and Yeast are present in the medium in elevated concentrations